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Objective:In order to explore the possibility of expanding the surgical indications of the ambulatory laparoscopic transcystic common bill duct exploration(LTCBDE).Methods:A retrospective case-control study analysis was performed on the clinical data of 585 patients who underwent laparoscopic transcystic common bill duct exploration in the Beijing Friendship Hospital Affiliated to Capital Medical University from January 2015 to December 2018, including 155 cases of the ambulatory LTCBDE (study group) and 430 cases of the overnight LTCBDE (control group). The propensity score was used to match the related variables, and 144 pairs were successfully matched. The two groups were compared in surgery-related indicators, such the preoperative tests, history of acute exacerbation in the last one month, history of upper abdominal surgery, concomitant diseases (high blood pressure, diabetes mellitus, coronary heart disease, cerebrovascular disease and respiratory diseases), american society of anesthesiology (ASA) score, intraoperative bleeding, operation time, total medical costs, unplanned readmission rate. Measurement data with normal distribution were expressed as mean±standard deviation( ± s), chi-square test was used for comparison between groups, and M( Q1, Q3) was used for measurement data with non-normal distribution. Fisher exact probability test and Wilcoxon rank-sum test were used for comparison between groups. Results:The indexes of aspartate aminotransferase (AST), alanine aminotransferase(ALT), total bilirubin, direct bilirubin (DBIL), alkaline phosphatase (ALP), gamma glutamyl transpeptidase (GGT), ASA score, intraoperative bleeding and total medical costs of the study group were 21.8(18.0, 31.0) U/L, 21.5(15.0, 42.5) U/L, 14.2(11.2, 18.6) umol/L, 2.6(2.1, 3.5) μmol/L, 91.0(73.3, 118.5) U/L, 44.5(22.0, 109.8) U/L, 2.0(2.0, 2.0) grade, 7.5(5.0, 10.0) mL, 9 659.0(8 704.8, 10 640.2) yuan respectively, and the control group were 26.0(17.7, 45.3) U/L, 29.5(16.0, 85.5) U/L, 17.2(13.0, 27.5) μmol/L, 3.8(2.4, 8.7) μmol/L, 105.0(81.0, 156.0) U/L, 86.5(25.0, 224.8) U/L, 2.0(2.0, 2.0) grade, 10.0(6.3, 20.0) mL, 14 366.6(11 766.4, 16 764.4) yuan, which the differences between the two groups were statistically significant ( P<0.05). In two groups of the surgery-related indicators, such as the history of acute exacerbation in the last one month, history of upper abdominal surgery, hypertension are significant statistical differences ( P<0.05). Then there were no significant differences in diabetes, coronary heart diseases, cerebrovascular disease, respiratory diseases, surgical time and unplanned readmission rate. Conclusion:The concomitant diseases may not be the absolute contraindications of the ambulatory LTCBDE. More patients can be treated with ambulatory LTCBDE in experienced centers if relevant outpatient assay and examinations can be further improved, risks of surgery and anesthesia can be fully evaluated, and surgical contraindications can be clearly excluded.
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Neuroendocrine tumors of the gallbladder(GB-NET) are rare, and it lacks early clinical manifestations and has no specific tumor markers, it is difficult to distinguish GB-NET from gallbladder adenocarcinoma. The diagnosis of GB-NET is based on histopathology of the tumor and the assessment of proliferation fraction, which makes it difficult to achieve early diagnosis. GB-NET has a high degree of malignancy, 32.39% of patients have liver metastases at diagnosis, and 51.60% of patients have lymph node metastases, the median survival time is 9 to 10 months.There are currently no specific guidelines or consensus for the treatment of GB-NET. The treatment strategies are choosen mainly by the principles of gallbladder adenocarcinoma. We reviews the clinical and basic researches of GB-NET and case reports from China and across the world, as well as the data from SEER database, and we discuss the research progress on the classification, clinicopathological features, diagnosis, treatment advances and the prognosis.
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Objective:To study the effect of PRR13 expression and the sensitivity of colorectal cancer cells to oxaliplatin, as well as the effect of PRR13 expression on the apoptosis rate of colorectal cancer cells under the action of oxaliplatin. Methods:Two colorectal cancer cell lines(SW620, SW480) were screened from the colorectal cancer cell lines commonly used by ATCC. Lentiviral vectors were used for cell transfection to construct a PRR13-silent colorectal cancer cell model. MTT oxaliplatin drug sensitivity experiments were conducted and compared. In this study, a colorectal cell model stably transfected with shRNA lentivirus containing the silenced PRR13 sequence was defined as the silent group, and a colorectal cell model stably transfected with empty vector of lentivirus was defined as the control group. After silencing the PRR13, the colorectal cancer cells changed the IC 50 of oxaliplatin, and then used a double staining flow cytometry apoptosis experiment to compare the expression of PRR13 and the rate of apoptosis of colorectal cancer cells under the action of oxaliplatin relationship. The measurement data were expressed as mean±standard deviation ( Mean± SD), and the comparison between groups used independent sample t test. The count data were expressed as percentage (%), and the comparison between groups used chi-square test. Results:The IC 50 of the same cell model decreased significantly with time. At the same time point, the IC 50 of the silent group was significantly lower than that of the control group. In the same cell model with the same concentration, the apoptosis rate of the silent group was significantly higher than that of the control group (SW620 silent group 22.5% vs SW620 control group 11.35%, SW480 silent group 13.63% vs SW480 control group 4.59%), the ratio of viable cells was significantly lower than that of the control cell model (SW620 silent group 76.0% vs SW620 control group 87.2%, SW480 silent group 74.5% vs SW480 control group 89.3%). Conclusion:Silencing the PRR13 can reduce the sensitivity of cells to oxaliplatin and reduce the drug resistance of oxaliplatin and the effect of treatment is more significant.
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Objective To analysize the clinicopathologyic features of intraductal papillary neoplasm of the bile duct (IPNB),aiming to increase clinic reorganization of it.Methods Nine patients with IPNB were treated at Department of General Surgery,Beijing Friendship Hospital,Capital Medical University from April 2009 to March 2019,including 5 males and 4 females,aged from 53 to 72 years old.All patients' clinical characteristics,diagnostic methods,treatment and prognosis were retrospectively analyzed.All the patients were followed up from 1 to 119 months (medium 31 months).Results The pathologic diagnosis of all the 9 cases were obtained.Seven cases had occured recurrent abdominal painand fever and 2 cases presented painless jaundice.Five patients had single lesion (4 cases at the distal of common bile duct,1 cases at the hilum),whereas 4 cases had multiple lesions (2 cases diffuse lesionsin the biliary tree,2 cases multicentriclesionsin the common bile duct).Eight patients were underwent surgical procedure,including 7 cases of pancreticoduodenectomy and 1 cases radical resection of hilar cholangiocarcinoma.One patient with diffuse lesions was treated by chemotherapy after pathologic diagnosis was confirmed.One was died at 88 months after surgery,others were survival.Conclusions IPNB is a rear disease.The multicentric lesions have more chances to progress to invasive lesions.Different operative approaches should be chosen for different tumor sites and extents.
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Objective To compare clinical outcomes between modified invagination pancreaticojejunostomyandmucosa (MIP) to mucosa duct pancreaticojejunostomy(MDP) in pancreaticoduodenectomy.Methods In Department of General Surgery,Beijing Friendship Hospital Capital Medical University,from Jan.2013 to Jan.2019,there were 222 cases performed pancreaticoduodenectomy,with MIP or MDP.According to the operation methods,by matching the sex and year decads,all patients were divided into MIP group (n =111) and MDP group (n =111).The incidence of pancreatic fistula in two groups was observed.The software of SPSS 20.0 was adopted for statistical analysis.Results There was no perioperative death in both groups.The mean operative time of MIP group was significant shorter than that of the MDP group [(212.8 ± 62.4) min vs (231.5 ±51.9) min,P =0.016].However,there were no significant differences in blood loss,blood transfusion,bowel fnnction recovery and liquid diet time.There was no grade C pancreatic fistula in MIP group,but 2 cases in MDP group.There were no significant differences in incidence and classification of pancreatic fistula between two groups.Conclusions There were no significant differences in clinical outcomes between MIP and MDP.The MIP is more easier to performed,so it is of certain spreading value.However,the further evidence need more prospective controlled study.
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Objective To investigate the safety and feasibility of laparoscopic common bile duct exploration through cystic duct while cholecystectomy by means of day surgery. Methods The data of69 patients who received day surgery of laparoscopic cholecystectomy and intraoperative transcystic biliary duct exploration from January 2015 to January 2018 in Beijing Friendship Hospital, Capital Medical University were analyzed. Preoperative symptoms and signs, laboratory examinations, imaging examinations, intraoperative exploration results, operation time, postoperative time, hospitalization expenses, as well as short time follow-up data were analyzed retrospectively. Results Sixty-nine cases of day surgery were iniolled.21.7％(15/69) were indicated in bile duct exploration according to medical history, and 33.3％ (23/69) simply because of abnormal blood examination which all showed elevated gamma-glutamyl transferase and 5.8％(4/69) only for positive imaging results. In the surgery, 24.6％(17/69) found bile duct stones, and 10.1％ (7/69) found sand-like stones or floccule in the biliary tract exploration, while negative exploration in 65, 2％(45/69). The operation time ranged from 41 to 169(median 111) minutes. No patient placed drainage tube in the surgery. Only 1 patient discharged at the first day after surgery because that nausea and vomiting led to a conversion to be hospitalized. The other patients discharged from the hospital in 1.4 to 8.3 (median 2.8) hours after the surgery; hospitalization costs 7 820 to 16 285 (Median 13 619) yuan. Telephone follow-up found no serious complications 3 days after operation, and1 patient complainted low grade fever 7 days after discharge due to gallbladder fossa fluid was cured by antibiotics. No residual stone was detected by ultrasound examination 3-month after surgery in follow-up cases. Conclusions As a practice of enhanced recovery after surgery concept, laparoscopic cholecystectomy and intraoperative transcystic bile duct exploration by means of day surgery is safe and feasible in experienced medical center. It could be safe when patients are strictly screened, although no effective method to predict the result of bile duct exploration by now.
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Objective To evaluate the optimal indications for the ambulatory laparoscopic cholecystectomy (ALC).Methods From Jan.2016 to Sep.2018,2277 case who underwent laparoscopic cholecystectomies were performed in Beijing Friendship Hospital,Capital Medical University,including 1072 cases of ALC (ALC group) and 835 cases of overnight laparoscopic cholecystectomy (OLC group).Indications including age,diagnosis,comorbidity and ASA score,and outcomes including operative time,analgesic,unplanned readmission and hospital cost were compared between ALC group and OLC group.Statistical analysis was conducted by the software of SPSS 20.0.Results There were no differences in the operative time [(42.6 ± 12.4) min vs.(48.7 ±20.3) min,P =0.326] and unplanned readmission (6/1072 vs.3/835,P =0.526).The examination cost [(1 667.10 ± 461.69) vs (3 156.44 ± 884.90)] and hospital cost (8 881.69 ± 1 954.34 vs.12 149.79 ± 3 476.59) was significant lower in ALC group,and the differences were statistically significant (P ＜ 0.01).Conclusions The comorbidities were not absolute exclusion criterion for ALC.More patients could be treated by ALC if the preoperative assessment streamlining in outpatient clinic was more improved.
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Objective To compare the clinical curative effect between mini laparoscopic cholecystectomy (MLC) and conventional laparoscopic cholecystectomy (LC).Methods Using random number table method to divide the patients into conventional laparoscopic cholecystectomy group (30 cases) and mini laparoscopic cholecystectomy group (27 cases).Compared the operation time,bleeding volume,VAS score,postoperative hospital stay,complications.Results No conversion to laparotomy and serious complications,but each group has 6 cases from the three holes increase to four holes,and 4 cases of MLC group were converted to traditional LC.MLC group and LC group,average operation time (57.5 ± 17) min and (50 ± 15) min (P ＞ 0.05);the average postoperative hospital stay was (2.5 ± 1.4) d and (3.1 ± 1.5) d (P ＞ 0.05);postoperative pain degree of MLC group of 6 h and 12 hrespectively (4.8±2.6) and (3.5±1.1),LC group were (5.5 ±2.7) and (5.0±2.1) points (P＜ 0.05).Conclusion Postoperative pain of MLC is lighter than LC,in appropriate cases,is a safe and effective treatment method.
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<p><b>OBJECTIVE</b>To evaluate the comparability and bias of the test results of two detection systems for serum procalcitonin (PCT) under the same laboratory condition.</p><p><b>METHODS</b>According to the profile NCCLS-EP9-A, the two systems were used to detect PCT to obtain the correlation coefficient and the liner equation for evaluation of the test result bias.</p><p><b>RESULTS AND CONCLUSION</b>The test results of PCT showed no significant difference between the two detection systems (P>005) with a kappa value greater than 0.75. The correlation coefficients of both systems were above 0.975, suggesting a consistency between them for clinical detection of PCT.</p>